IFNB1 and Reoviridae infectious disease: As a third complementary strategy to assess the impact of IFNs on the first round of T3D reovirus replication, we treated L929 cells with IFNβ at various timepoints pre- and post- reovirus infection, and monitored cytokine expression (S1A Fig), virus titers (S1B Fig), or virus protein expression (S1C Fig) at 18hpi.