We used three antibodies for this assay: a rabbit monoclonal antibody that recognizes the C-terminal HA tag; a mouse monoclonal antibody that recognizes an epitope upstream of UIM1-2 (1H9); and a rabbit polyclonal antibody generated against full-length ataxin-3 (Machado-Joseph disease [MJD]; Paulson et al., 1997). The gene discussed is ATXN3; the disease is Machado-Joseph disease.