There was no significant enrichment in neurons expressing the Pomc gene, a neuropeptide-encoding gene with known coding mutations causing monogenic obesity in humans (4/5 of Pomc+ cell populations were nominally enriched; HYPR-NEURO24 (Pomc/Ttr), p=0.002; ARCME-NEURO21 (Pomc/Glipr1), p=0.01; HYPC-NEURO23 (Pomc/Cartp), p=0.03; HYPR-NEURO24 (Pomc), p=3.0×10−3). Here, NPS is linked to obesity due to melanocortin 4 receptor deficiency.