Multivariate analysis also showed that higher methylation of CDC5L-cg05671347, RHOA-cg05657651 and YBX1-cg16306148, and lower methylation of BCR-cg25410636, DFFA-cg20696875, SCUBE2-cg19000089 and TP63-cg06520450, were independent prognostic factors for OS in HCC patients. This evidence concerns the gene CDC5L and hepatocellular carcinoma.