Taken together, our results show that DNA methylation expressions of CDC5L-cg05671347, RHOA-cg05657651, YBX1-cg1630414, BCR-cg25410636, DFFA-cg20696875, SCUBE2-cg19000089 and TP63-cg06520450 were independent prognostic factors for OS in HCC patients. This evidence concerns the gene SCUBE2 and hepatocellular carcinoma.