CNGB3 and achromatopsia: The CRISPR-Cas9 system has emerged as a revolutionary tool for generating loss-of-function mutations.19, 20, 21, 22 In the present study, we generated a CNGB3 somatic knockout model in NHPs to recapitulate achromatopsia through subretinal injection of adeno-associated virus 9 (AAV9) with CRISPR-Cas9 targeting CNGB3. This new model exhibited a reduced amplitude in the central retina, as shown by multifocal electroretinogram (mfERG).