In this study, we generated a somatic CNGB3 knockout model in NHPs to recapitulate achromatopsia through subretinal injection of AAV-mediated CRISPR-Cas9 targeting CNGB3. The indel ratio detected in deep sequencing was low, and we speculate there are two possible explanations: (1) the component of the AAVs in the area of retina dissected for deep sequencing was poorly enriched in the central macula, or (2) in contrast to immunohistochemistry and single-cell sequencing in DsRed+ cones, deep sequencing was performed with a large number of cells, including uninfected cells. The gene discussed is CNGB3; the disease is achromatopsia.