Acute GVHD was induced in vivo by intravenous injection of C57Bl/6 splenocytes or tolerogenic cultures containing C57Bl/6 transfected DCs and autologous splenocytes into CBF1 (F1: CBA×C57BL/6) mice (Figure 5(a)). The gene discussed is RBPJ; the disease is graft versus host disease.