Here, we found that lncRNA OTUD6B-AS1 transcription was activated by As2O3 in bladder cancer cells, and these results were validated using a xenograft tumor model, suggesting that the antitumor effects of As2O3 may be via upregulation of lncRNA OTUD6B-AS1; however, the roles of lncRNA OTUD6B-AS1 in exacerbated As2O3-induced oxidative damage have yet to be elucidated. This evidence concerns the gene OTUD6B and urinary bladder cancer.