TNFSF10 and neoplasm: TRAIL is a small cytokine expressed by most cell types that selectively induces apoptosis in tumor cells overexpressing death receptors while sparing healthy cells.11,14 In this model, we successfully knocked in our gene of interest (GOI), expressing the chimeric protein eGFP-TRAIL, into the safe harbor site AAVS1 on chromosome 19 of the proto-neutrophilic cell line PLB-985 using the CRISPR/Cas system (Figs. 2a and 2b).