GFAP+ astrocytes present in the striatum and corpus callosum might be the main sources of IGF-1 expression in that area, in that GFAP+/IGF-1+ double-positive cells constituted up to 72.66 ± 8.98% of IGF-1+ cells in the ischemia group, which was significantly increased to 81.22 ± 9.70% in the MSC group (Figure 4(o)). Here, IGF1 is linked to ischemia.