The NRP‐1 protein expression positive rate was 100% in GBC tumours (40.7% positive and 59.3% strong positive), 36.7% in acute cholecystitis (63.3% negative, 30% positive and 6.7% strong positive) and 58.3% in chronic cholecystitis (41.7% negative, 48.3% positive and 10% strong positive) (P < .01, χ2 = 99.6), indicating the expression of NRP‐1 was significantly increased in GBC tumours (Figure 1A). The gene discussed is NRP1; the disease is Cholecystitis.