To examine whether autophagy collaborates with Sirt3 to counteract hematopoietic aging, we began with in vitro colony‐forming assays in which the Sirt3‐depleted HSC‐enriched cells, prepared by infection of the HSC‐enriched cells (GFP+) with a lentivirus vector bearing shRNA against the Sirt3 gene, were cultured in methylcellulose medium supplemented with growth factors. This evidence concerns the gene SIRT3 and infection.