In the absence of mechanical forces, comparisons of CDH LOs with those derived from normal (non‐CDH) controls revealed impaired expression of NKX2.1+ lung progenitors, type II alveolar epithelial cells, and platelet derived growth factor receptor‐alpha+ (PDGFRα) myofibroblasts. The gene discussed is PDGFRA; the disease is congenital diaphragmatic hernia.