After further adjustment for preoperative comorbidities (hypertension, type 2 diabetes, coronary heart disease, chronic obstructive pulmonary disease), preoperative analgesic use, and preoperative hemoglobin and albumin based on model 1, using logistic and linear regression analyses tourniquet use was still correlated with a higher transfusion rate (RR = 2.319, 95%CI 1.841–2.922, P < 0.001) and longer PLOS (B = 1.278, 95%CI 1.061–1.495, P < 0.001) (model 2). This evidence concerns the gene ALB and chronic obstructive pulmonary disease.