Moreover, exogenous addition of pyruvate to spheroid medium significantly reduced ROS generation and increased P-glycoprotein expression, which was conversely suppressed by iodoacetate or 2-deoxy-d-glucose; these findings indicate that MDR phenotype of PCa tumoroids is closely related to the glycolytic metabolism of cancer cells and can be abolished by glycolysis inhibitors via mechanisms that involve changes in the cellular redox state [62]. Here, ABCB1 is linked to cancer.