Similarly, DCs loaded with KG-1 or primary AML cells also induced a significant up-regulation of PD-1 MFI on suppressive Tregs to 3.9 ± 0.3 or 4.0 ± 0.3, respectively (fold change), after Flu treatment compared to un-loaded DCs (p < 0.05 or p < 0.01, respectively) (Figure 8C). This evidence concerns the gene PDCD1 and acute myeloid leukemia.