KRT20 and colorectal carcinoma: In total, this study compared the detection of CTC in the blood of CRC patients by the use of three methods: (1) IF staining and semi-automated microscopical enumeration by the cell imager NYONE®; (2) isolation via the ScreenCell® Cyto IS device coupled with cytochemistry according to Pappenheim and IF staining followed by microscopical enumeration; (3) semi-quantitative CK20 RT-qPCR.