Overall, the above findings suggest that LINC00473 weakened radiation therapy’s effect in ESCC patients via the cumulative activity of miR-374a-5p/miR-497-5p/SPIN1/PARP1; therefore, LINC00473 could act as a novel prognostic biomarker and a potential therapeutic target for ESCC. Here, SPIN1 is linked to esophageal squamous cell carcinoma.