Additionally, statistical analysis revealed that AFAP1-AS1 could efficiently distinguish between ESCC samples and healthy esophageal mucosa (79.4% specificity and 73.3% sensitivity) and could also detect early ESCC (stage I + II, n = 79) with a specificity and sensitivity of 92.3% and 44.6% respectively [21]. This evidence concerns the gene AFAP1 and esophageal squamous cell carcinoma.