To determine the role of the TLR4/MyD88/NF-κB pathway in puerarin-mediated inhibition of the inflammatory response, we studied the activities of TLR4, MyD88, p-NF-κB, and TNF-α in adipose tissues around the reproductive organs in GDM rats by western blotting to elucidate the precise molecular mechanism of the anti-inflammatory activity of puerarin. This evidence concerns the gene TLR4 and gestational diabetes.