ESR1 and Familial prostate cancer: To identify drug-pairs for the detailed time course RNA-seq analysis, we leveraged a pre-existing LINCS drug combination dataset collected at Columbia University (Califano’s lab) in the MCF7 cell line, a breast cancer line that is ERα positive (Lee et al., 2015), and the LNCaP cell line, a prostate cancer cell line that is ERβ positive (Takahashi et al., 2007).