In this study, we investigated whether HPV16 E6 downregulated the expression of p‐LKB1 through the HPV‐KIF7‐LKB1 axis, and report that HPV16‐E6 but not E7 inhibited the antitumor activity of LKB1 in lung cancer cells by downregulating the expression of KIF7. Here, STK11 is linked to lung cancer.