We examined neurofilament (NF) as it is found in protein aggregates of several neurodegenerative diseases (reviewed in Didonna & Opal, 2019), including ALS (Mendonça et al, 2005) and was readily detected by Western blot in the starting brain lysates while we could not detect TDP‐43 in the same, possibly due to low amount. Here, TARDBP is linked to amyotrophic lateral sclerosis.