Among the 53 genes, PLK1, integrin-linked protein kinase (ILK), transformation/transcription domain-associated protein (TRRAP), PKMYT1, and TP53RK were hits in all 3 cell lines, and PLK1 and ILK have been reported to be oncogenes in osteosarcoma. The gene discussed is PKMYT1; the disease is osteosarcoma.