Ki67 staining of lymph nodes confirmed the metastatic lesions (Figure 3C), and the analysis of primary tumor metastasis to inguinal lymph nodes (as quantified by the area of human Ki67-positive signal, determined by using anti-human Ki67 antibody, which does not cross react with mouse Ki67) showed an approximately two-fold lower rate of metastasis in the anlotinib group than the control group (Figure 3D). This evidence concerns the gene MKI67 and neoplasm.