Fortunately, Maunakea et al. previously analyzed the genome-wide distribution of MeCP2 in colon cancer cell line HCT116 (2013, GSE47678) by ChIP-seq, which helped us to avoid the problem of screening primers to identify the exact target DNA fragments for PCR detections (Additional file 1: Fig. S2).Using HAUS8 gene as a MeCP2 positive control [23], the results identified the binding of MeCP2 to opn exon4 and exon5 in HCT-8 cells at native states (Fig. 6a). The gene discussed is MECP2; the disease is malignant colon neoplasm.