Real-time PCR was performed on cDNA from 57 CaEs tumor and adjacent normal tissues, expression of RUNX3 mRNA was found to be significantly increased in tumors (5.056 ± 5.331, relative values to β-actin expression) compared with normal tissue (5.603 ± 5.709 relative values to β-actin expression) (p < 0.04) (Fig. 1). The gene discussed is ACTB; the disease is neoplasm.