Through our research, we found that miR-221-3p can bind to KIT, CDKN1B, RUNX2, and BCL2L11 in PC cells and that the expression of miR-221-3p can affect KIT, CDKN1B, RUNX2, and BCL2L11 expression. This evidence concerns the gene CDKN1B and pachyonychia congenita.