To illustrate the regulatory mechanism of KLF5 in CRC, we obtained the target genes of other TFs based on 5500 ChIP-Seq profiles from the Cistrome database [37] and proposed a two-step enrichment analysis method to identify candidate TFs that may co-operate with KLF5 (Additional file 1: Figure S1). The gene discussed is KLF5; the disease is colorectal carcinoma.