ACAN and Severe short stature: Since ACAN-D2 was found in heterozygosity in one Mangalarga Marchador horse, despite the small number of sampled animals, we suggest that the potential association between ACAN:c.6465A > T (RefSeq XM_005602799.2—EquCab3.0) and dwarfism in Miniature horses would continue to be relevant; moreover, ACAN:c.6465A > T has provided population, genetic, and informatics prediction evidence that it could be a potential causative variant of dwarfism in this breed22.