The genotyping for the four previously described causative variants (D1, D2, D3* and D4) of dwarfism2 in the 18 dwarf Miniature horses used in this study revealed that 13 animals were heterozygous for only ACAN-D4 (N/D4), two animals were heterozygous for only ACAN-D2 (N/D2), one animal had the D2/D3* genotype, and two animals did not possess any of the known dwarfism alleles (D1, D2, D3*, or D4). This evidence concerns the gene ACAN and Severe short stature.