However, at 90 days post-transplantation, the frequency and an absolute number of BCR-ABL1/EGFP+ LSK cells had decreased significantly in Lgr4Gt/Gt retro-CML mice compared with Lgr4+/+ retro-CML mice (Fig. 7f; Supplementary Fig. 13b). Here, LGR4 is linked to chronic myelogenous leukemia, BCR-ABL1 positive.