Flow cytometry following a t-SNE (t-Distributed Stochastic Neighbour Embedding) algorithm analysis indicated that PB samples from untreated control and Gdpd3-deficient retro-CML-affected mice both contained BCR-ABL1/EGFP+ leukaemia cells that were distinct from the populations of normal myelogenous cells, T cells and B cells in PB of normal healthy WT mice (Supplementary Fig. 9a, b, e). This evidence concerns the gene GDPD3 and leukemia.