We first harvested bone marrow mononuclear cells (BMMNCs) from littermate healthy control mice (SCL-tTA+) and tet-CML mice (SCL-tTA+TRE-BCR-ABL1+), and isolated LT-stem cells (CD150+CD48−CD135−LSK), CD48+LSK cells, multipotent progenitors (MPP), and LK (Lineage−Sca-1−cKit+) populations (Supplementary Fig. 2). Here, BCR is linked to chronic myelogenous leukemia, BCR-ABL1 positive.