JAG1 and breast cancer: The in vitro experiments showed that the siRNA-mediated linc00514 knockdown in breast cancer cells reduced the protein level of pSTAT3 and Jagged1, and that the pcDNA-mediated linc00514 overexpression in breast cancer cells increased the protein level of pSTAT3 and Jagged1 (Fig. 3b-i & b-ii).