Using a single cycle infection assay, we blocked the replication-competent NL4-3_eGFP virus with protease inhibitor Darunavir, and sorted the non-productively-infected and uninfected (eGFP-) and productively-infected (eGFP+) CD4+ T-cell populations from CD4/CD8 co-cultures or CD4 mono-cultures three days post infection to assess: (i) the amount of integrated HIV-DNA, (ii) the frequencies of inducible provirus by reactivation of sorted eGFP-CD4+ T population, and (iii) the number of HIV transcripts on a per-integrated-provirus basis. The gene discussed is CD4; the disease is infection.