While our experimental system did not directly address the impact of activated CD8+ T cells in the establishment and maintenance of HIV latency in CD4+ T cells, it did not escape our attention that this non-cytolytic activity of CD8+ T cells may increase the in vivo pool of latently infected CD4+ T cells under ART and therefore represent a key, previously unrecognized obstacle to the elimination of the virus reservoir and the eradication of HIV infection. This evidence concerns the gene CD4 and HIV infectious disease.