We showed here that mice treated with 19 or 38 mg/kg NBP MNP 6 hours before renal ischemia or mice treated with 38 mg/kg NBP MNP 15 minutes after reperfusion were protected against ischemic AKI compared with control MNP-treated mice, as demonstrated by reduced plasma creatinine and BUN as well as kidney NGAL mRNA expression (N = 5–7). Here, LCN2 is linked to acute kidney injury.