CCl4-exposed HSCs of rats with cirrhosis cultured on high stiffness matrices and treated with 50 μM liraglutide (a drug with described antifibrotic effects)21 showed a significant decrease in the mRNA markers of HSC activation α-SMA and collagen I. This downregulation, however, was not observed in cells cultured on low stiffness matrices (Fig. 5), indicating that stiffness could influence the response to pharmacological antifibrotic strategies. Here, ACTA1 is linked to Cirrhosis.