To assess the synaptic damage due to HIV-infection in viable neurons, we further examined the intensity of staining of PSD-95 and Synaptophysin (SYN) immunolabeled puncta (Fig. 7D) on day 30 of MG-hBORGs culture and plotted the mean intensity of each puncta as a fraction of Tuj-1 maximum intensity (Fig. 7E,F). Here, SYP is linked to HIV infectious disease.