Similarly, IPA analysis of BioLayout 3D clusters with progressive or similar changes at 3 and 7 months showed RAC signalling, Axonal guidance, Rho family GTPase signalling, Stathmin1 regulation of breast cancer and ARP-WASP complex1 to be significantly altered (p = 7.09E−10, 1.19E−09, 2.91E−09, 3.84E−08, 1.03E−07, respectively) (Supplementary Figure S4B, Supplementary Table S11). The gene discussed is AKT1; the disease is breast carcinoma.