At 48 h of infection, Mtb ΔRD1 infection did not significantly upregulate IFN, TREM1 and TNFR2 signalling (Fig. 2F), and there were fewer dysregulated genes in immune pathways after Mtb ΔRD1 infection when compared to Mtb WT (Fig. 2F). Here, TREM1 is linked to infection.