By contrast, according to the known T2D-related miRNA target or pathway genes (such as p38, PTEN, PI3K/AKt, Bax, Foxa22, and Glut4) [52, 53], we found that none of these genes were screened probably because the short-term glucose-stimulated culture cannot rapidly induce changes in gene expression. This evidence concerns the gene PTEN and type 2 diabetes mellitus.