For example, to 1) engage costimulatory molecules, e.g., with expression of CD40L/CD154; 2) enhance cross-presentation of tumour antigens e.g., with heat shock proteins; 3) enhance APC function, e.g., with agonists of TLRs; 4) reduce immune suppression in the TME [261]. The gene discussed is CD40LG; the disease is neoplasm.