To clarify whether the untested effectors might be putative Avr genes, we performed cDNA PenSeq for the four P. infestans isolates EU_13_A2, EC1_A1, EU_6_A1, and US23, at different time points after infection (12 hr postinoculation [hpi], 1, 2, and 3 dpi) and in mycelium and zoospores (Figure 1b). This evidence concerns the gene NLRP6 and infection.