To begin to address the pathophysiological implications of Sema3A/NRP1 expression in ENS developmental disorders, we assessed the protein expression of Sema3A and its receptor NRP1 in the ganglionic sigmoid colon of HSCR patients, considered by pathologists as the healthy segment, compared to the sigmoid colon of a group of patients without HSCR but with anocolorectal malformation (ARM). Here, NRP1 is linked to Hirschsprung disease.