The irg-1p::gfp reporter worms were challenged with a Pseudomonas aeruginosa strain isolated from natural habitats harboring wild C. elegans populations, which has been shown to perturb mitochondrial function and induce expression of the UPRmt reporter hsp-6p::gfp2. In wild-type animals, irg-1p::gfp expression was induced upon Pseudomonas infection, whereas deficiency of hda-1 significantly suppressed the induction of irg-1, to a similar extent as deficiency of dve-1 (Fig. 3a; Supplementary Fig. 4a, b). Here, ACOD1 is linked to Pseudomonas infection.