To assess whether the GPI anchor or the hydrophobic stretch at the C terminus is required for the function of ALPP in ZIKV infection, we constructed an ALPP-C-terminus-deleted protein (ALPP-ΔC33) and an ALPP-D484W mutant that rendered a loss of the GPI linker (Fig. 4C). The gene discussed is ALPP; the disease is Zika virus infectious disease.