Genes in the same chromatin domains containing T-ALLgained CTCF binding sites, especially those genes with higher expression in T-ALL compared to normal CD4+ T cells, were significantly affected by CTCF silencing (odds ratio = 11.3, P = 4.6e−7) (Fig. 6f), indicating that T-ALLgained CTCF sites are the most disrupted in our silencing study. This evidence concerns the gene CD4 and acute lymphoblastic leukemia.