Using publicly available ChIP-seq data, we examined the activating histone marks H3K4me1, H3K27ac, and the repressive mark H3K27me3 and found that cancer-specific gained CTCF binding associates with increased levels of enhancer marks H3K4me1 and H3K27ac (P < 0.001 by t-test), while lost CTCF sites correlate with decreased level of these two enhancer marks (P < 0.05 by t-test) (Additional file 1: Fig. S6). The gene discussed is CTCF; the disease is cancer.