Huang et al. showed S. miltiorrhiza and ligustrazine injection (SLI), prepared in a proportion of 1.2:60, had a beneficial effect on myocardial ischemia/reperfusion (I/R) and hypoxia/reoxygenation (H/R) injuries in mice subjected to coronary artery occlusion and 2 h reperfusion via Akt serine/threonine kinase (Akt)-endothelial nitric oxide synthase (eNOS) signaling pathway [57]. This evidence concerns the gene NOS3 and myocardial ischemia.