WNT3A and melanoma: Figure 4B shows that cinobufagin-mediated suppression of Wnt/β-catenin target genes was abolished in LEF1 knocked-down A375 melanoma cells. LEF1 knock-down efficiency was confirmed (Figure 4C). In addition, cinobufagin suppressed Wnt3a-induced canonical Wnt signaling-related genes expression in human melanocytes (Figure 4D).