Interestingly, we found that cinobufagin decreased nuclear LEF1 levels in a dose-dependent manner in A375 (Figure 3A), G361 (Figure 3B), and A2058 (Figure 3C) melanoma cells, indicating that cinobufagin may attenuate canonical Wnt/β-catenin signaling by decreasing LEF1 expression in melanoma. The gene discussed is LEF1; the disease is melanoma.