To distinguish between the effect of the MM cell-derived soluble angiogenic factors and the MM cell-derived Jagged-mediated activation of the angiogenic Notch signaling in the HPAECs, we set up a 24 h tube formation assay stimulating the HPAECs with soluble Jagged1 and Jagged2 peptides. Here, JAG1 is linked to Miyoshi myopathy.