To further verify the regulation roles of FOXA1 on DSCAM-AS1, we knocked down FOXA1 by siRNAs in lung adenocarcinoma, breast cancer, and prostate cancer cell lines, respectively, qPCR and Western blotting showed these siRNAs could effectively silence FOXA1 levels. This evidence concerns the gene DSCAM-AS1 and lung adenocarcinoma.