In order to investigate the signals representing different TNFR1-complexes under TNF-α stimulation in HCC cells, we detected effectors of TNFR1-complex I (phopsho-P65/P65 and IκB) and TNFR1-complex II (cleaved Caspase-8/Caspase-8 and FADD) in SMMC-7721 cells exposed to TNF-α via western blotting. The gene discussed is FADD; the disease is hepatocellular carcinoma.