Based on the differences in PRL-3 expression observed in the HCC cell lines, we chose Huh7 and Hep3B to establish cell lines stably overexpressing PRL-3, while the cell lines SK-Hep-1 and HepG2 were used for PRL-3 knockdown experiments (Figure 3A; Figure S2B). Here, PTP4A3 is linked to hepatocellular carcinoma.