BxPC-3 cells were cultured for a long time under normal or acidic conditions to further reveal the effects of the Myc/MTSS1-AS pathway on metastasis of PC in vivo and then stably transfected with lentivirus-containing negative control (Lv-NC), or MTSS1-AS (Lv-MTSS1-AS), or co-transfected with Lv-MTSS1-AS and lentivirus-containing Myc (Lv-Myc). The gene discussed is MTSS1; the disease is pachyonychia congenita.